Ls23l sequence analysis
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Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
Ls23l sequence analysis full#
Both are based on in vitro DNA synthesis b. Depositing Scientist Sequences: Full (1) Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results.
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With the exception of Zm Atg5, for which we were unable to generate a cDNA by reverse transcription (RT)-PCR, presumably due to the high GC content of its 5 end, cDNAs encompassing the full coding region for each. The in vitro DNA amplification technique, the polymerase chain reaction (PCR), has facilitated the use of these probes by greatly increasing the number of copies.
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1 Virology and Pathogenesis Group, Microbiology Services Division, Public Health England, Wiltshire, United Kingdom Department of Pathology and Infectious Disease, School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom Wildlife Zoonoses and Vector-Borne Diseases Research Group, Animal and Plant Health Agency, Addlestone, Surrey, United Kingdom 2 Department of Pathology and Infectious Disease, School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom. Our maize gene models were further validated by sequence analysis of a collection of transcripts for each Zm Atg gene (Table II). The analysis of DNA for the presence of particular mutations or polymorphisms can be readily accomplished by differential hybridization with sequence-specific oligonucleotide probes.